anyone here????? help me please!!!

[Lyfroker]

how do we test a broad bean for its protein content?
i knw the test with biuret reagents bt hv sm confusion, cn any1 explain it to me in detail?

and also oct/nov 2004 p6 Q1 (a)

 

[Lyfroker]

Re: biology

no reply yet...........

 

[Bouchra]

@Lyfroker:

I remember reading in the textbook about how to test food for protein content.

You have to chop up the food (in this case the broad bean), and put it in a test tube with some water. Then add some potassium hydroxide solution and 2 drops of copper sulfate solution.

Then shake the tube. If a purple color appears, then protein is present.


Hope this helps you.

Bouchra

 

[Bouchra]

You may also want to check out this Wikipedia article: http://en.wikipedia.org/wiki/Biuret_test

It covers more about the same topic -- seems to be advanced stuff but may be of help to you = )