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Biology Practical

maissa93

maissa93

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Hey guys. This is my first post here and i hope that my queries might be answered. I'd be highly obliged..

Can anyone please tell me the general topics for biology practical that is due tomorrow? As in like the first question has enzymes, food tests and ?? So for the second question..

I'd be SO grateful.. Thanks a lot in advance.. (=
 
maissa93

maissa93

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That's what I'm asking too.. I'm so not sure about them. Since there is no syllabus outline or any such thing.. =/
 
C

Coldsmmer

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well there was something about urease!!
enzymes!!
and nov 1999 p3 and p5!!
do u have it??
nov 1999 p3 and p5?
 
maissa93

maissa93

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umm hmm.. I don't have those papers.. can you please send me the link or something?
 
maissa93

maissa93

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Also, can you please tell me how to make the calculations? That is the EPG and the Stage micrometer thingy?
 
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filza94

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hey m new here can sum1 help me n send those papers too me as m realli worried watz going to cum...........
 
ammu2011

ammu2011

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Hey all.. Yhe 4 2mrw We might have Urease... N Transverse Section of leaf.. :) Hopefully..
 
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yoyo123

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why is everyone asking for nov 1999??? i mean, i can't find it anywhere. but what's so special about that paper anyways?
 
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filza94

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Ok Duh we're getting urease & urea tomorrow in Bio 33, Now what wud be the Improvements, Sources of error & Limitations for the experiment below?
Enzyme Concentration

In this investigation, we will examine what happens to the rate of a reaction when the amount of enzyme is reduced. We will use urease, an enzyme that converts urea to ammonia. The ammonia causes the pH of the water to increase (it becomes more basic). You will be able to tell when a reaction occurs because the urea solution also contains a pH indicator that is becomes yellow in acid but turns red when the solution becomes basic.

The object of this experiment is to measure the amount of time it takes for the solution to turn red if less enzyme is used.

C1. Create a hypothesis regarding the the amount of urease and the rate of reaction of Urea.

C2. Obtain four test tubes and add 2 cm of urea to each.

C3. Label three of these tubes 1 through 3; the remaining tube will not be used; it will serve as a control.

C4. Have your lab partner start timing as you add 15 drops of urease to tube #1 and then swirl the tube until it changes to a red color. Record the amount of time that it took for the urease to change to a red color.

C5. Add 5 drops of urease to tube #2 and then swirl the tube until it changes to a red color. Record the amount of time that it took for the urease to change to a red color.

C6. Add 1 drop of urease to tube #3 and then swirl the tube until it changes to a red color. Record the amount of time that it took for the urease to change to a red color.

C7. Record your results in the answer sheet.

C8. Did using less enzyme produce a reaction?

C9. What was the effect of using less enzyme in your experiment? If your experiment did not work as expected, what should have happened?

C10. In general, what happens to the rate of reaction as the amount of enzyme is decreased?

C11. Do your results support your hypothesis? Explain.
 
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