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dude thnk you =) but i dont get how at high temp theres leakage nd at the same time high absorbtion rate ??See wen u hold the beetroot too tightly with the forceps it causes pinching of the membrane resulting in leakage of pigment at low temperatures as the tonoplast in damaged.At low temperatures the rate of diffusion or leakage decreases or stops as the molecules of phospholipid have a low kinectic energy and as the membrane is still intact and at high temperature the molecules of the phospholipid moves with a higher kinetic energy thus resulting in the membrane becoming more fluid and as the rate of diffusion increases thus pigment leaks out. Also, denaturation of membrane proteins may play a part. Yes, and high temperature and increasing conc. of ethanol both increase the % absorbance.