protein denaturation

[sarah_faith]

How do acids denature proteins?
Does the pH of the acid affect the results?
Answer needed ASAP!!!

 

[Anika Raisa]

How do acids denature proteins?
Does the pH of the acid affect the results?
Answer needed ASAP!!!

Proteins such as enzymes are affected by pH.Some enzymes work best at pH below 7 some above 7. It depends on the type of enzyme. If an enzyme suitable to work at pH above 7 is introduced to acidic condition, it will be danatured. pH change will affect and disrupt the secondary and tertiary structures of the protein, changing the protein's shape. As the function of the protein depends on it's shape (catalytic activity), it will lose its function.
So the active site of the enzyme loses is shape and so the substrate can no longer bind to the active site.
(an acid environment will usually denature the bonds that link the proteins.)

Or Alternate:


How does a change in ph affect the structural levels of a protein?

pH can affect the structure of proteins, because the standard structure for a protein is,

N-C-(R)-C=O The R in the middle represents an R group that is attached to the middle carbon. The right-most C is attached to the middle carbon also.

In general, there are only 3 places that you want to look to see how pH affects the structural levels of a protein. Proteins consists of polypeptides, which are basically polymers of amino acids.

The ends of the protein are not connect to each other, and have pKa values which mean they can be protontated/deprotonated depending on the pH of the environment that the protein is in. Secondly, the R-groups may also have a pKa depending which amino acid they are, and thus will alter whether those R-groups will be protonated or deprotonated. In turn, the protonation/deprotonation of some of these groups will result in formation of positive charges, negative charges or neutral charges which will play a role in electrostatic interactions when the protein folds.

Hope i could be helpful !

 

[Anika Raisa]

For high level explanation visit: http://class.fst.ohio-state.edu/FST822/lectures/Denat.htm

 

[sarah_faith]

Proteins such as enzymes are affected by pH.Some enzymes work best at pH below 7 some above 7. It depends on the type of enzyme. If an enzyme suitable to work at pH above 7 is introduced to acidic condition, it will be danatured. pH change will affect and disrupt the secondary and tertiary structures of the protein, changing the protein's shape. As the function of the protein depends on it's shape (catalytic activity), it will lose its function.
So the active site of the enzyme loses is shape and so the substrate can no longer bind to the active site.
(an acid environment will usually denature the bonds that link the proteins.)

Or Alternate:


How does a change in ph affect the structural levels of a protein?

pH can affect the structure of proteins, because the standard structure for a protein is,

N-C-(R)-C=O The R in the middle represents an R group that is attached to the middle carbon. The right-most C is attached to the middle carbon also.

In general, there are only 3 places that you want to look to see how pH affects the structural levels of a protein. Proteins consists of polypeptides, which are basically polymers of amino acids.

The ends of the protein are not connect to each other, and have pKa values which mean they can be protontated/deprotonated depending on the pH of the environment that the protein is in. Secondly, the R-groups may also have a pKa depending which amino acid they are, and thus will alter whether those R-groups will be protonated or deprotonated. In turn, the protonation/deprotonation of some of these groups will result in formation of positive charges, negative charges or neutral charges which will play a role in electrostatic interactions when the protein folds.

Hope i could be helpful !

thank you so much

 

[Anika Raisa]

My pleasure...