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no one does.can someone please explain the experimental evidence for the semi-conservative replication of DNA in genetics. I dont understand the whole heavy DNA and the generations stuff...
Evidence for the semi-conservative replication of DNA.
In an experiment on e.coli(a bacteria) these were used to follow the DNA in the new cells every generation.
NOTE : Bacteria are used in this experiment since they replicate very rapidly causing this experiment not to last too long.(Generation time is about 50 minutes at 36oC)
The bacteria were grown in a nutrient medium containing the heavy isotope of Nitrogen-15. This replaced the normal Nitrogen-14 in the DNA. The bacteria labelled with N-15 contained the original or parental DNA. These bacteria were then transferred in a medium containing N-14 and allowed to grow.
Other similar bacteria were left for their DNA to replicate 2 or 3 times. The DNA was then extracted and centrifuged. The heavier the DNA, the closer to the bottom of the tube it settles. The DNA appeared as bands in the tubes as shown.