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AS BIOLOGY P-33
- Thread starter beeloooo
- Start date
- Messages
- 50
- Reaction score
- 17
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- 18
alrite guys... got my hand on it....
Question 1
test upon sucrose, glucose, starch and fungal amylase...... u need to knw how to distinguish b/t reducing sugars n non reducing sugars....
Question 2
measurement of some part of plant... i cldnt knw exactly wat so sorry...but slide will be provided n v will ve to observe it under microscope...
cheers guys... do remembr me in ur prayers...its least i can ask frm u... can i?
yar what is the function of that "fungal amylase".i have never performed a test on it
- Messages
- 2,113
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- 982
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- 123
amylase? enzyme? protien? v dun have to worry bout its function...just have to distinguish em.....
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To Hydrolyse starch to convert it into reducing sugar so we can perform benedicts test on it.(thats what i know though)
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THANKS
- Messages
- 50
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- 17
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thanx buddy!!!!!!!!!
buddy!!!!!!!!!
- Messages
- 2,113
- Reaction score
- 982
- Points
- 123
it protien man....not starch?
alrite guys... got my hand on it....
Question 1
test upon sucrose, glucose, starch and fungal amylase...... u need to knw how to distinguish b/t reducing sugars n non reducing sugars....
Question 2
measurement of some part of plant... i cldnt knw exactly wat so sorry...but slide will be provided n v will ve to observe it under microscope...
cheers guys... do remembr me in ur prayers...its least i can ask frm u... can i?
great job
thanks falcon
- Messages
- 2,113
- Reaction score
- 982
- Points
- 123
- Messages
- 22
- Reaction score
- 10
- Points
- 13
S
Amylase + starch -----> Maltose
Starch is included in the reagents right?
Amylase + starch -----> Maltose
- Messages
- 2,113
- Reaction score
- 982
- Points
- 123
v will have to use the other method...as v need to test enzyme also...for all v knw it cld be denatured due to the heat n stuff...so v ve to be sure...so use the other method....
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- 306
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- 278
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- 38
simply it is enzyme amylase
- Messages
- 306
- Reaction score
- 278
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- 38
What I heard is: we have to perform Benedict test on the known solutions of Glucose and than we have to change any variable for enzyme to check the hydrolysis of starch
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- 22
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- 10
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- 13
Whats the other method ? )
- Messages
- 24
- Reaction score
- 11
- Points
- 13
thank u so oooooooooooooooo much u r a life saveralrite guys... got my hand on it....
Question 1
test upon sucrose, glucose, starch and fungal amylase...... u need to knw how to distinguish b/t reducing sugars n non reducing sugars....
Question 2
measurement of some part of plant... i cldnt knw exactly wat so sorry...but slide will be provided n v will ve to observe it under microscope...
cheers guys... do remembr me in ur prayers...its least i can ask frm u... can i?
- Messages
- 24
- Reaction score
- 11
- Points
- 13
confused...
- Messages
- 2,113
- Reaction score
- 982
- Points
- 123
Whats the other method ? )
add HCl n heat in to hydrolise starch.....then add NaHCO3 to neutrilize HCl added befre..then perfrm the normal reducing sugar test....
umm fungal amylase is protien?
- Messages
- 113
- Reaction score
- 17
- Points
- 28
Amylase is an enzyme, right? So it is made up of proteins.add HCl n heat in to hydrolise starch.....then add NaHCO3 to neutrilize HCl added befre..then perfrm the normal reducing sugar test....
umm fungal amylase is protien?