BIO PPR 3 HELP NEEDED

[Adahshan]

I lost my paper about genetic engineering of insulin. Can anyone give me it?

Thanks!

 

[adityar]

What do you mean???

 

[Cozmo35d]

Here this will help ya.

Link : http://www.abpischools.org.uk/res/coRes ... ticeng.cfm

By the way the insulin gene is cut from a human chromosome by an enzyme named restriction enzyme, and this newly cut gene is inserted into a plasmid by using the ligase enzyme, so if they asked you how to make genetic engineering you have to mention those two enzymes and what they do.

I hope that helped you, and good luck with your exam

 

[Adahshan]

Can you give me all steps written?

 

[Cozmo35d]

Can you give me all steps written?

I had it written on my copybook, but it's lost , thank god ive got the study guide to help me instead.

 

[Adahshan]

Damn, i need the whole steps written ;/ PLEASE

 

[XxMarkxX]

hey ! i need to know this question too !! can anybody give an organised answer pls !!! with steps i mean !!!

 

[Adahshan]

Gene of interest is isolated by a restriction endonuclease enzyme.
The bacterial plasmid is also isolated and cut open by the same restriction endonuclease enzyme.
Gene is then taken and placed inside the opening of the plasmid using ligase enzyme.
The recombinant plasmid is then taken and placed inside the bacteria, its now called transgenic bacteria.
Bacteria starts to divide forming insulin.

There gl mark

 

[Cozmo35d]

Gene of interest is isolated by a restriction endonuclease enzyme.
The bacterial plasmid is also isolated and cut open by the same restriction endonuclease enzyme.
Gene is then taken and placed inside the opening of the plasmid using ligase enzyme.
The recombinant plasmid is then taken and placed inside the bacteria, its now called transgenic bacteria.
Bacteria starts to divide forming insulin.

There gl mark

Exactly