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I had it written on my copybook, but it's lost , thank god ive got the study guide to help me instead.Adahshan said:Can you give me all steps written?
ExactlyAdahshan said:Gene of interest is isolated by a restriction endonuclease enzyme.
The bacterial plasmid is also isolated and cut open by the same restriction endonuclease enzyme.
Gene is then taken and placed inside the opening of the plasmid using ligase enzyme.
The recombinant plasmid is then taken and placed inside the bacteria, its now called transgenic bacteria.
Bacteria starts to divide forming insulin.
There gl mark
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