paper chromatography.....relies on partition...in which the substance is partitioned between water trapped b/w cellulose fibres and the mobile solvent......ASA,
Can anyone explain the chromatography thing? like what is the stationary phase? what is the mobile phase? what are the two types of seperation techniques used in chromatography, like adsobtion and partition?
I have read it many times in the support booklet and in Chemguide but still can't understand it.. any help would be greatly appreciated!
TLC uses adsorption mechanism....it is usually a kind of tile .....whose surface has lots of OHs sticking out from the silica compound it is actually made...of so substance cn form boonds of varying strength with this or get dissolved in mobile phase and get carried upward......u shud memorise marksccheme definition of PARTITION and adsorption......as for glc.....we have an inert gas such as helium as the carrier gas....and carbowax....a non polar hydrocarbon as the stationary phase.....the same is tru for HPLC...bt u dont have a carrier gas....rather u have a liquid under hogh pressure as the carrier........u shud knw wat does retention time means....and be able interpret the glc graph and that area represent the proportion of particular substance present in the mixture