Physics, Chemistry and Biology: Post your doubts here!

[NIM]

for chemistry paper6 is it necessary to learn much of the theory part?

no not really!!!
u just need to hav basic information...

 

[always-smile :)]

guys plllz i have a question
explain why and outline how human insulin genes were put into bacteria using genetic engineering ??
can anybody help

 

[sma786]

I've got a much better site x)
http://www.youtube-mp3.org/

mabrook, u win this tym xP

 

[ZainH]

guys plllz i have a question
explain why and outline how human insulin genes were put into bacteria using genetic engineering ??
can anybody help

First the gene for human insulin is isolated using a restriction-endonuculease enzyme (A enzyme which basically cuts genes) . Then a bacterial plasmid from a bacterial cell is also cut using the SAME restriction-endonuclease enzyme. This produces sticky ends (Meaning that they are now complimentary and will join) . Lignin (A polysaccharide) acts as glue and joins the sticky ends to each other. The bacterial plasmid is then returned to the bacterial cell which is then placed in a fermenter for mass production.

 

[natsume hyuga]

First the gene for human insulin is isolated using a restriction-endonuculease enzyme (A enzyme which basically cuts genes) . Then a bacterial plasmid from a bacterial cell is also cut using the SAME restriction-endonuclease enzyme. This produces sticky ends (Meaning that they are now complimentary and will join) . Lignin (A polysaccharide) acts as glue and joins the sticky ends to each other. The bacterial plasmid is then returned to the bacterial cell which is then placed in a fermenter for mass production.

lignin ? isnt it the enzyme ligase?

 

[natsume hyuga]

btw are there any flame tests we need to know to be able to identify the group 1 metals?

 

[always-smile :)]

First the gene for human insulin is isolated using a restriction-endonuculease enzyme (A enzyme which basically cuts genes) . Then a bacterial plasmid from a bacterial cell is also cut using the SAME restriction-endonuclease enzyme. This produces sticky ends (Meaning that they are now complimentary and will join) . Lignin (A polysaccharide) acts as glue and joins the sticky ends to each other. The bacterial plasmid is then returned to the bacterial cell which is then placed in a fermenter for mass production.

thnx alot

 

[ZainH]

lignin ? isnt it the enzyme ligase?

LOL, I can't beleive I wrote lignin.. it is ligase, my bad :s

thnx alot

Your welcome!

 

[IGSCE.Geek.]

hey guys can i ask you something....when dealing with graphs and they ak to drawa best fit line....HOw do u do it?

 

[sumangupta]

Outline how enzymes can be manufactured for use in biological washing powders?

 

[ZainH]

hey guys can i ask you something....when dealing with graphs and they ak to drawa best fit line....HOw do u do it?

Just join the first point the the last point. Or just make a line in the middle of all your points plotted.

Outline how enzymes can be manufactured for use in biological washing powders?

Basically you place a bacteria or a fungus in a fermenter and provide it with a food source such as glucose. Since fungus make extracellular digestion , they will secreate their digestive enzymes (Protease, lipase, carbohydrase) onto the food source. These enzymes can later be collected from the fermenter. This was a brief answer, just try to explain fermentation while answering such a question.

 

[sumangupta]

sure....thanks alot

 

[ZainH]

sure....thanks alot

Anytime x)

 

[always-smile :)]

Outline how enzymes can be manufactured for use in biological washing powders?

we put food stock ( sugar + ammonia + fungi + bacteria ) with pumping air inside the ferminter for aerobic respiration of the micro organismms and pass water current around the fermenter to keep temp. constant inside. Micro organisms will produce their enzymes then suck the solution and pass it on filter. Yhe filtrate contains lipase and protease enzymes which can be extracted

 

[IGSCE.Geek.]

Guys, can someone explain to me HIV and how it effects the immune system (extended) [please?

 

[natsume hyuga]

anyone flame colours that we need to know in chemistry to identify a metal?

 

[always-smile :)]

anyone flame colours that we need to know in chemistry to identify a metal?

lithium ----> pink or crimson
sodium ----> yellowish orange
potassium ---> lilac or violet
hope i helped

 

[ZainH]

Guys, can someone explain to me HIV and how it effects the immune system (extended) [please?

HIV targets lymphocytes , specially T lymphocytes. These lymphocytes stimulate B cells to produce antibodies, as well as destroy pathogens them selves. HIV destroys these lymphocytes , meaning B cells will not be able to produce anti bodies to fight pathogens. This WEAKENS our immune system causing us to be more prone to diseases and infections.

 

[beewhiz997]

Can I have the notes please?

sure here :http://www.xtremepapers.com/communi...cs-chemistry-biology-notes.14611/#post-258146

 

[beewhiz997]

thnx may ALLAH BLESS YOU

youre welcome hope the notes helped!