- Messages
- 510
- Reaction score
- 607
- Points
- 103
So you mean that during cold denaturation bonds do not break?At low temperature, reaction rate is low because collision between enzymes and substrate is not effective. The collision rate is low as well. When temperature increases, the bonds that maintain the three dimensional shape of the enzyme start to break and get affected, so enzyme shape changes, too high a temperature and it will be denatured permanently. The bonds that break are probably hydrogen bonds.