why the test tube is washed with tap water, then with distilled water and then with hydrogencarbonate indicator to investigate gaseous exchange during photosynthesis, i cant get the logic behind in washing the testube with three diff solutions
Tap water contains contaminents. We make sure that there are no contaminents left by washing with distilled water. And the hydrogen carbonate is used to check for presence of CO2 as it's yellow in presence of CO2 and red in absense.