- Messages
- 100
- Reaction score
- 35
- Points
- 38
independent= concentration of subtilisin A
dependent = rate of removal of gelatin
i don't exactly remember the independent
-
We need your support!
We are currently struggling to cover the operational costs of Xtremepapers, as a result we might have to shut this website down. Please donate if we have helped you and help make a difference in other students' lives!
Click here to Donate Now (View Announcement)
Biology P51
- Thread starter Senorita.K
- Start date
dependent = rate of removal of gelatin
i don't exactly remember the independent
- Messages
- 45
- Reaction score
- 8
- Points
- 8
yup i wrote the same
- Messages
- 45
- Reaction score
- 8
- Points
- 8
- Messages
- 150
- Reaction score
- 166
- Points
- 53
What did you guys write in the dilution question?? Please answer ? Was it serial or proportional ?
- Messages
- 100
- Reaction score
- 35
- Points
- 38
I used proportional. Since serial dilution could produce very dilute solutions. But I don't think they'll judge that..? They asked for a procedure, not a method, if i'm not wrong.
- Messages
- 45
- Reaction score
- 8
- Points
- 8
it can be both and yes as Komail said they asked for a procedure so you can write either about serial dilution or about proportional dilution
but i wrote about serial dilution
- Messages
- 45
- Reaction score
- 8
- Points
- 8
for the first question,the first part (a) about the problems with measuring the time,i think, what did you write ?
- Messages
- 85
- Reaction score
- 135
- Points
- 43
did you convert from mg to ug in this question
- Messages
- 100
- Reaction score
- 35
- Points
- 38
never came across my mind :0!
- Messages
- 85
- Reaction score
- 135
- Points
- 43
i even forgot to do this step i just did the normal serial dilution and used the formulae c1v1=c2v2
how did u do it
- Messages
- 150
- Reaction score
- 166
- Points
- 53
I wrote that we will use serial dilution and did the halfing method (decrease the conc by half each time ) is this correct ?
- Messages
- 85
- Reaction score
- 135
- Points
- 43
i did the same but not the halfing method
i made conc 0.8, 0.6, 0.4, 0.2
i made conc 0.8, 0.6, 0.4, 0.2
- Messages
- 100
- Reaction score
- 35
- Points
- 38
same. but i did not use any formula.
I gave proportional values for 5 solutions.
- Messages
- 85
- Reaction score
- 135
- Points
- 43
like how proportional i don't get it
- Messages
- 100
- Reaction score
- 35
- Points
- 38
like 50cm3 of subtilisin A
40cm3 of subtilisin A, 10 cm3 of EDTA and saline buffer solution
and so on..
- Messages
- 85
- Reaction score
- 135
- Points
- 43
- Messages
- 100
- Reaction score
- 35
- Points
- 38
was it um per gram? i don't remember it.oh yeah i did the same
do you think we will lose marks for not converting
anyway, the original solution was in um3 and in a bottle of cm3.. dealing with um is simpler than cm (in this case).. but idk though
- Messages
- 45
- Reaction score
- 8
- Points
- 8
i dont think so we had to do any conversion
- Messages
- 45
- Reaction score
- 8
- Points
- 8
when you are using serial dilution method you dont have to use any formula
c1v1=c2v2 formula is used for normal dilution(dilution other than serial dilution) , you can never use this formula for serial dilution because serial dilution means just halfing the concentration
- Messages
- 85
- Reaction score
- 135
- Points
- 43
ooops then i wrote something and explained sth else *facepalmed* i hope they go blind while reading this :/when you are using serial dilution method you dont have to use any formula
c1v1=c2v2 formula is used for normal dilution(dilution other than serial dilution) , you can never use this formula for serial dilution because serial dilution means just halfing the concentration
the conversion part i mean is as i remember they said we have a 1 mgcm-3 subtilisin
and we need to prepare different conc ranging from 20 ugcm-3 - 100 ugcm-3
i forgot doing this and just made conc 0.8, 0.6, 0.4, 0.2 all in mgcm-3