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independent= concentration of subtilisin A
dependent = rate of removal of gelatin
i don't exactly remember the independent
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Biology P51
- Thread starter Senorita.K
- Start date
dependent = rate of removal of gelatin
i don't exactly remember the independent
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yup i wrote the same
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What did you guys write in the dilution question?? Please answer ? Was it serial or proportional ?
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I used proportional. Since serial dilution could produce very dilute solutions. But I don't think they'll judge that..? They asked for a procedure, not a method, if i'm not wrong.
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it can be both and yes as Komail said they asked for a procedure so you can write either about serial dilution or about proportional dilution
but i wrote about serial dilution
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for the first question,the first part (a) about the problems with measuring the time,i think, what did you write ?
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did you convert from mg to ug in this question
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never came across my mind :0!
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i even forgot to do this step i just did the normal serial dilution and used the formulae c1v1=c2v2
how did u do it
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I wrote that we will use serial dilution and did the halfing method (decrease the conc by half each time ) is this correct ?
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i did the same but not the halfing method
i made conc 0.8, 0.6, 0.4, 0.2
i made conc 0.8, 0.6, 0.4, 0.2
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same. but i did not use any formula.
I gave proportional values for 5 solutions.
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like how proportional i don't get it
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like 50cm3 of subtilisin A
40cm3 of subtilisin A, 10 cm3 of EDTA and saline buffer solution
and so on..
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was it um per gram? i don't remember it.oh yeah i did the same
do you think we will lose marks for not converting
anyway, the original solution was in um3 and in a bottle of cm3.. dealing with um is simpler than cm (in this case).. but idk though
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i dont think so we had to do any conversion
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when you are using serial dilution method you dont have to use any formula
c1v1=c2v2 formula is used for normal dilution(dilution other than serial dilution) , you can never use this formula for serial dilution because serial dilution means just halfing the concentration
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ooops then i wrote something and explained sth else *facepalmed* i hope they go blind while reading this :/when you are using serial dilution method you dont have to use any formula
c1v1=c2v2 formula is used for normal dilution(dilution other than serial dilution) , you can never use this formula for serial dilution because serial dilution means just halfing the concentration
the conversion part i mean is as i remember they said we have a 1 mgcm-3 subtilisin
and we need to prepare different conc ranging from 20 ugcm-3 - 100 ugcm-3
i forgot doing this and just made conc 0.8, 0.6, 0.4, 0.2 all in mgcm-3