Paper Discusion: Bio/52 & /51

[sumeru]

No Hydrogen bonds aint so weak to be broken by just 60^C :/

i wrote that temperature is used to denature the nucleus, so that DNA can flow out....

 

[Malik777]

i wrote that temperature is used to denature the nucleus, so that DNA can flow out....

one student from my center also wrote the same ;p

 

[sumeru]

one student from my center also wrote the same ;p

what do u think might be the gt?

 

[Malik777]

what do u think might be the gt?

would be 20 :/
But I wish it would be lesser :/

 

[sumeru]

would be 20 :/
But I wish it would be lesser :/

yes...

 

[LeQuavina]

I wrote PH of the soil and nitrogen concentrationfor the abiotic factors. Is it wrong?

 

[hadi6512462]

Malik777 Fire?

 

[Zarif009]

Hey

Answers: (I can be wrong)

a)i) To hydrolyse lipids as detergents contain Enzymes Lipase.

ii) To Denature the enzymes

iii) To seperate the Debris of peas from the DNA mixture.

iv) To hydrolyse proteins associated with DNA like histone.

b) After treating with Restriction enzymes, place the 3 samples on three different agrose Gel. place it at Cathode(-ve) and apply the potential difference. The fragments will move to the anode, depending on their size/mass. smaller mass fragment will move the farther and larger will move the smallest distance. Use the DNA probes to identify the Fragments./markers. You can use neuliotide containing Radioactive phosphate. The complimentary neucliotides would combine with each other. and DNA bands are visible on the photographic film. Repeat this at least thrice. This experiment is a low risk/ electric shock and its precaution.

c)i) Numbers of different size of DNA fragments

ii) Temperature and concentration/volume of restriction enzyme.

d)i) Number of Bands for REsEnzi are greater than the bands from ResEnz22.

ii) DNA treated with both of the restriction enzymes is having more number of bands than the respective samples treated with only one restriction enzyme.

iii)Insufficient samples there should be one sample which is first treated with REsEnzi and then with Res Enz 2.

d)i) I started from the right end of the electrogram and used the complimentary bases.

Hey bro. For d) iii) i wrote that some of the fragments are not distinguishable from the others. The sharpness of the image is too low. Will that be accepted?

 

[Malik777]

Malik777 Fire?

I am waiting bro :'D

 

[IbtiCool]

Both the probes and DNA fragments have complementary sequences, you're not wrong! You'll get a mark for that, don't sweat.
Example: the light is off. That means it's dark. You could also say: it's dark here. Which means that the light is off. Both are right, right?

And don't worry. If you don't get full marks for that, you will get minimum 3.

Haha yeah but I'm not really interested in Bio. I just gave it for some reason... It was very stupid of me LOL I'm just focusing on Chem, Phy and Maths. Plus the exam schedule this time is very messed up

 

[Oishee Asif]

Haha yeah but I'm not really interested in Bio. I just gave it for some reason... It was very stupid of me LOL I'm just focusing on Chem, Phy and Maths. Plus the exam schedule this time is very messed up

It is messed up :\
Regardless, getting a better grade is always better than a worse grade

 

[Igcse stuff]

Just so you know, I'm a complete idiot. Warning given.

Electro:

1. RNA probes are either radioactive or fluorescent to mark the specific DNA fragments, and select them thereafter. The DNA samples are cut using restriction enzymes, forming double-stranded fragments. These are heated and treated such that single-stranded fragments are left with sticky ends, which are complementary to the RNA probes. The RNA probes are single-stranded, and when these probes are mixed with the fragments, they are selected.

2. As the slightest change in the sequence can be picked up (single change in one nucleotide), this could be used for genetic screening. Frame shifts, silent mutations, base deletions, substitutions, additions can be found, blah blah...

3. I wrote so much, my hand broke.

4. pH, voltage.

5. I drew a horizontal line through A, so all my arrows were in line with that. The one that cut through A had all 4 varieties.

6. Varieties 1 & 2.

Sorghum: *swears internally*

1. Normal distribution. Continuous data.

2. I messed this question up.

3. Messed this up too.

4. Time (1 month) left before experiment began, forgot the other one

5. Conc of nitrate ions already present in soil, amount of water in soil

6. I think I wrote:
From table 2.2, overall plant showed significant differences when treated with both roots and shoots, the diff was not due to chance; from table 2.2, roots showed no significant difference; from table 2.1, mean dry mass increased for whole plant when used with any treatment.

7. 44.1%, 3

8. OH and null hypothesis: The use of green manure had no effect on the sorghum or something like that ;_;;;;

(And hehe. Wrote 'Electro'. Spidey's enemyyyy. Such a nerd I am. Lalala)
http://static2.hypable.com/wp-conte...azing-spider-man-2-villain-electro-poster.jpg

Can this apply to Chem P4?

 

[Oishee Asif]

Can this apply to Chem P4?

For Section B?
Yeah.