- Messages
- 958
- Reaction score
- 3,499
- Points
- 253
For w08
From Fig 3.1, we have 2 categories of alleles of the gene
Now for membrane protein alleles, IA and Ialook at the fig)
- membrane protein allele
- muscle protein allele
For muscle protein allele:
- IA and Ia have equal frequency (you can easily count them)
- Ia is smaller and negatively charged. This is because, according to the technique of electrophoresis, the smaller fragments move faster towards they anode. (DNA is negatively charged phosphate backbone which are attracted towards the anode ie downwards) The smaller they are, they move faster and the more negatively charged they are.
Overall notice that muscle protein alleles are smaller (more negatively charged) than muscle protein allele
- Ima is the smallest
- Imb is the largest among muscle protein allele
hope i could help Soldier313
JazakAllah khair for that
But erm my doubt is that how can we determine which of the alleles are more negative without being provided with the direction of movement of the fragments?