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Biology; Chemistry; Physics: Post your doubts here!

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how to prepare a microscope slide..
for animal cell(10)
for plant cel(10)
basically eed to know thoroughly how to prepare a slide
 
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before statring ethanol emulsion test y do u make sure tht the ethanol test tube is dry...
and y is ethanol added before water....
 
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10 marks!!!
I saw it as a max of like 4 in the paper
This is for a plant cell

View attachment 40203
exploded diper here you go!
  1. First add a few drops of water or solution on the microscope slide to avoid dryness and wilting
  2. Take a small piece of onion and using forceps (tweezers), peel off the membrane from the underside (the rough side).
  3. Lay the membrane flat on the surface of the slide
  4. Using a pin, lower a thin glass cover slip or cover glass onto the slide. Make sure there are no air bubbles
  5. Looking from the side (NOT through the eyepiece), lower the tube using the coarse focus knob until the end of the objective lens is just above the cover glass. Do this carefully so as not to crack the cover glass (and possibly damage the objective lens)
  6. Now look through the eyepiece and turn ONLY the smaller, fine focusing knob to move the optical tube upwards until an image comes into focus. The cells should look something like lizard skin.
  7. Swap the objective lens for a higher powered one so that you can see the cells at greater magnification. You should be able to make out a nucleus in each cell.
  8. Repeat the process after adding a dye solution (iodine or methylene blue). Be very careful; these dyes can stain your skin and clothes.#copied. You may add that the section should be as thin as possible for clearer observation under the microscope.
 
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exploded diper here you go!
  1. First add a few drops of water or solution on the microscope slide to avoid dryness and wilting
  2. Take a small piece of onion and using forceps (tweezers), peel off the membrane from the underside (the rough side).
  3. Lay the membrane flat on the surface of the slide
  4. Using a pin, lower a thin glass cover slip or cover glass onto the slide. Make sure there are no air bubbles
  5. Looking from the side (NOT through the eyepiece), lower the tube using the coarse focus knob until the end of the objective lens is just above the cover glass. Do this carefully so as not to crack the cover glass (and possibly damage the objective lens)
  6. Now look through the eyepiece and turn ONLY the smaller, fine focusing knob to move the optical tube upwards until an image comes into focus. The cells should look something like lizard skin.
  7. Swap the objective lens for a higher powered one so that you can see the cells at greater magnification. You should be able to make out a nucleus in each cell.
  8. Repeat the process after adding a dye solution (iodine or methylene blue). Be very careful; these dyes can stain your skin and clothes.#copied. You may add that the section should be as thin as possible for clearer observation under the microscope.
thx man
 
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before statring ethanol emulsion test y do u make sure tht the ethanol test tube is dry...
and y is ethanol added before water....
The test tube must be dry because any water or chemicals in the test tube may affect the experiment and consequently the result
Ethanol emulsifies fats (fats are soluble in ethanol). If we added water first, then there would be no reaction. When the lipids dissolve in ethanol, they will precipitate in water
 
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