Thank youur a genius1. dilute the 10% penicillin to prepare various concentrations.
2. Soak the filter paper discs in different concentrations of penicillin
3. On the petri dishes where bacteria are growing, discs are placed after covering with lid, the plates are incubated at 37degrees for 24 hrs.
4. after 24 hrs the diameter of clear areas around discs is measured
5. Formation on clear areas determines the concentration of penicillin required to kill bacteria.